Diagnostic free Prostate Specific Antigen f-PSA test

Diagnostic Kit for free Prostate Specific Antigen (fluorescence immunochromatographic assay)

SUMMARY

Free prostate-specific antigen (fPSA) is a prostate-specific antigen released into the blood in a free form and secreted by prostate epithelial cells. PSA（Prostate Specific Antigen） is synthesized and secreted by prostate epithelial cells into semen and is one of the main components of seminal plasma.It contains 237 amino acid residues and its molecular weight is about 34kD.It has serine protease activity of the single chain glycoprotein,participate in the process of semen liquefaction. PSA in the blood is the sum of the free PSA and the combined PSA. the blood plasma levels, in 4 ng/mL for the critical value, the PSA in prostate cancer Ⅰ ~ Ⅳ period of the sensitivity of 63%, 71%, 81% and 88% respectivel.

PRINCIPLE OF THE PROCEDURE

The membrane of the test device is coated with anti fPSA antibody on the test region and goat anti rabbit IgG antibody on the control region. Lable pad are coated by fluorescence labeled anti PSA antibody and rabbit IgG in advance. When testing positive sample, the fPSA antigen in sample combine with fluorescence labeled anti PSA antibody, and form immune mixture. Under the action of the immunochromatography, the complex flow in the direction of absorbent paper, when complex passed the test region, it combined with anti fPSA coating antibody, forms new complex. fPSA level is positively correlated with fluorescence signal, and the concentration of fPSA in sample can be detected by fluorescence immunoassay assay.