Monkeypox Virus DNA Detection Kit (Fluorescent Real Time PCR Method)

            Monkeypox Virus DNA Detection Kit

            (Fluorescent Real Time PCR Method)

SUMMARY

The monkeypox virus is the causative pathogen of monkeypox, which is a viral zoonotic disease with symptoms in humans similar to those seen in smallpox patients. Early symptoms include fever, headache, muscle pains, fatigue, and swollen glands. After a few days of early symptoms, patients may develop a few to thousands of characteristic lesions on the face, hands, and feet. The monkeypox virus can be found in the patients’ body fluid and contaminated belongings and spread by close contact with contaminated items.

PRINCIPLE THE DETECTION

The Monkeypox Virus (MPV) DNA Detection Kit (Fluorescent Real Time PCR Method) uses the real time polymerase chain reaction method to detect MPV DNA fragments in body fluid samples from suspected patients. The PCR Buffer provided in the kit contains a pair of specific PCR primers to amplify the MPV DNA fragment, and a taqman probe that bind to amplified DNA fragment to monitor the reaction progress in real time. Besides, the buffer also includes an external control system to monitor presence of inhibitory substances.

Test procedures

 Read the Package Insert Carefully Prior to First Use. Equipment and Instruments Required but Not Provided in This Product.

1. Real time PCR system
2.  Real time PCR reaction tubes/plates
3. Viral DNA extraction kit
4. Vortex mixer
5. Mini centrifuge with rotor for centrifuge tubes.
6. Biohazard waste container.
7. Pipettes and pipette tips
8. Disposable gloves
9. Safety goggles

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