Monkeypox Virus IgG/IgM Antibody Test

            Monkeypox Virus IgG/IgM Antibody Test

SUMMARY

Monkeypox is a viral zoonosis (a virus transmitted to humans from animals) with symptoms very similar to those seen in the past in smallpox patients, although it is clinically less severe. With the eradication of smallpox in 1980 and subsequent cessation of smallpox vaccination, monkeypox has emerged as the most important orthopoxvirus for public health. Monkeypox primarily occurs in Central and West Africa, often in proximity to tropical rainforests and has been increasingly appearing in urban areas. Animal hosts include a range of rodents and non-human primates. Monkeypox virus is an enveloped double-stranded DNA virus that belongs to the Orthopoxvirus genus of the Poxviridae family. After a person is infected with MPV for the first time, the body's immune system will carry out immune defense against the virus and produce specific antibodies, and IgM antibodies will be produced in the body of acute infection 3~7, which mainly exist in the acute infection period. A few days later, IgG antibodies began to appear and increase, and the presence in the body was longer than that of IgM antibodies, with better affinity, suggesting the presence of previous infection.

PRINCIPLE THE DETECTION

Monkeypox Virus IgG/IgM Antibody Test is a lateral flow chromatographic immunoassay for the qualitative detection of MPV IgG/IgM antibody in human serum or plasma. If the specimen contains MPV IgG antibody, it will bind with the colloidal gold labelled MPV antigen to form a sandwich complex. As the anti-human IgG antibody is adsorbed on the test line (T1 line), colloidal gold will accumulate and precipitate here and appear red. If the specimen contains MPV IgM antibody, it will bind with the colloidal gold labelled MPV antigen to form a sandwich complex. As the anti-human IgM antibody is adsorbed on the test line (T2 line), colloidal gold will accumulate and precipitate here and appear red. To serve as a procedural control, a colored line will always appear in the control line (C line) region, indicating that proper volume of specimen has been added and membrane wicking has occurred.

Test procedures

 Read the Package Insert Carefully Prior to First Use. Equipment and Instruments Required but Not Provided in This Product.

Please read this manual carefully before use. Please follow the procedures below. For serum or plasma:

1) Tear off the seal of aluminum foil bag and carefully take out the test device.

2) Transfer 1 drop (about 10μL) of serum or plasma with the disposable plasticdropper into the sample well of the test device. 3) Add 2 drop of the diluent and leave it at ambient temperature. Start the timer. Interpret the result within 10~15 minutes. Do not read the result after 15 minutes

INTERPRETATION OF TEST RESULTS

IgG/IgM POSITIVE (+): Three distinct colored line appears. One line in the control line region (C line) and two lines in the test line region (T1 line and T2 line). The shade of color may vary，but it should be considered positive whenever there is even a faint line.

IgG POSITIVE (+): Two distinct colored line appears. One line in the control line region (C line) and the other line in the test line region (T1 line), indicating that the MPV IgG antibodies were detected in the specimen.

IgM POSITIVE (+): Two distinct colored line appears. One line in the control line region (C line) and the other line in the test line region (T2 line), indicating that the MPV IgM antibodies were detected in the specimen.

IgG/IgM NEGATIVE (-): Only one colored line appears in the control line region, and no colored line appears in the test line region.

INVALID: The Control line (C line) fails to appear. The test is invalid even if a colored line appears in the test line region. Insufficient sample volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the test procedure and repeat the test using a new test device. If the problem persists, discontinue using the product immediately and contact your local distributor.

More related products for  monkeypox:

https://www.baysenmedical.com/monkeypox-virus-dna-detection-kit-fluorescent-real-time-pcr-method_p223.html

https://www.baysenmedical.com/monkeypox-virus-antigen-test-rapid-test-kit_p222.html